🤑 Recombinant Anti-DNA:RNA hybrid antibody [S] (ab) | Abcam

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Antibody [ENH] Anti-DNA-RNA Hybrid [S] Antibody by Kerafast. Sciences on 25 January by Chang, W. F., Xu, J., et al.. Applications: IHC. Effects of.


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DNA-RNA Hybrid antibody (S): ChIP-Seq, DB, IF, EMSA
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s9 6 antibody immunofluorescence

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The monoclonal antibody S is a widely-used tool to purify, analyse and quantify R-loop structures in cells. A previous study using the surface.


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This mouse monoclonal antibody was generated against a ΦX bacteriophage-derived synthetic DNA–RNA antigen and recognizes RNA-DNA hybrids of.


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To characterize R-loops in fission yeast, we used the S antibody-based DRIPc​-seq method to sequence the RNA strand of R-loops and.


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s9 6 antibody immunofluorescence

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and S antibody was conserved at ° C in aliquots (after thawing, each aliquot was immediately S stocks were also titrated with immunofluorescence.


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s9 6 antibody immunofluorescence

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This mouse monoclonal antibody was generated against a ΦX bacteriophage-derived synthetic DNA–RNA antigen and recognizes RNA-DNA hybrids of.


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s9 6 antibody immunofluorescence

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6, Cat. No. MABE, is a highly specific mouse monoclonal antibody, that targets DNA-RNA hybrid and has been tested in Affinity Binding Assay, Chromatin.


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s9 6 antibody immunofluorescence

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Mouse recombinant monoclonal DNA:RNA hybrid antibody [S]. We do not recommend using this antibody for imaging applications such as ICC-IF, IHC or.


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s9 6 antibody immunofluorescence

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IP with S antibody in the presence of mM RNA/DNA hybrid mulation as measured by immunofluorescence (IF) with the. S antibody.


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s9 6 antibody immunofluorescence

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6] Antibody has been validated for the following applications: Sequencing, EMSA​, Microarray, ChIP, Immunocytochemistry, Immunofluorescence.


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s9 6 antibody immunofluorescence

For example, in an affinity binding assay ABA , this antibody was shown to bind the DNA-RNA heteropolymer and poly I -poly dC equally, but fold higher levels of poly A -poly dT were required to achieve a similar degree of binding Boguslawski et al. Published Species Reactivity:. Close Menu. Published Application s :. They are shown to be resistant to the activity of DNA methyltransferases. DNA-RNA hybrids are a natural occurrence within eukaryotic cells and their level are high at sites of high transcriptional activity.

Immunol Methods. In the original publication by S9 6 antibody immunofluorescence et al. Characterization of monoclonal antibody to DNA. Their presence is reported to predispose a locus to chromosomal breakage. Application Notes: DNA-RNA hybrids are a natural occurrence within eukaryotic cells, with levels of these hybrids increasing at sites with high transcriptional activity, such as during transcription initiation, repression, and elongation.

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Clone Name:. Close Filter. Mutations in the DNA:RNA helicase senataxin SETX are implicated in the dominant juvenile form of amyotrophic lateral sclerosis type 4 and a recessive form of ataxia oculomotor apraxia type 2. Antibody first published in: Boguslawski et al. J Immunol Methods. RNA and its application to immunodetection of hybrids. They are non-canonical nucleic acid structures with transcriptional regulatory functions.